论文编号:SW157 论文字数:12590,页数:30 摘 要:考查了马克斯克鲁维酵母 AS2.1440 在有机溶剂/水两相系统中生物转化L-苯丙氨酸生产 β-苯乙醇的过程。在单因素试验的基础上,用Plackett-Burman 设计、最陡爬坡试验结合响应面分析法对影响β-苯乙醇的因素进行了研究,确定了最佳发酵条件为:两相比例控制在油酸/水=2 : 3为宜;装液量为64.46 mL/150mL三角瓶,培养基初始pH控制在5.0,温度32.99℃,转速160 r/min下转化48 h,β-苯乙醇的产量可达1533±11 mg/L。与传统水相相比,油酸-水两相系统中Kluyveromyces marxianus AS2.1440 转化 L-苯丙氨酸生产 β-苯乙醇的产量提高了 102.91%。 关键词:两相体系;β-苯乙醇;响应面方法; AS2.1440 Abstract: Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 was performed in an aqueous/organic solvent biphasic system. On the basis of single-factor tests, factors affecting Bioconversion of L-phenylalanine to β-phenylethylalcohol with Kluyveromyces marxianus AS2.1440 were evaluated by Plackett-Burman design, the path of steepest ascent and response surface methodology. The optimal bioconversion conditions are as follows: the concentration of L-phenylalanine was 4 g/L, the optimum values of volume ratio of organic phase to aqueous phase, reaction temperature, pH value and liquid volume were 2 : 3, 32.99℃, 5.0, and 64.46mL/250mL respectively. Under above optimum conditions, the content of β-phenylethylalcohol reached1533±11g/L48 h later, which increased by about 102.91% than that in monophasic aqueous system. eywords:biphasic system; β-phenylethylalcohol; Response Surface Methodology; Kluyveromyces marxianus AS2.1440 目 录 本站会员可自行下载: 响应面法优化两相体系发酵生产天然香料β-苯乙醇 (收费:12800 积分) 如何获取积分?
响应面法优化两相体系发酵生产天然香料β-苯乙醇相关论文
|
本论文在生物工程论文栏目,由工程论文网整理,转载请注明来源 www.gcLunwen.top 更多论文,请点工程管理论文查看 | |
上一篇:嘌呤啤酒酵母选育 | 下一篇:羊肚菌菌核形成影响因素 |
推荐论文 | 本专业最新论文 |
点击查看关于 响应 优化 体系 发酵 生产 天然 香料 乙醇 的相关论文题目 | 2011-03-31 20:34:38【返回顶部】 |